Serum protein electrophoresis by CZE 2000 clinical capillary electrophoresis system
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چکیده
منابع مشابه
Serum protein electrophoresis by CZE 2000 clinical capillary electrophoresis system.
We compared the automated Paragon 2000 clinical capillary zone electrophoresis (CZE) system with two manual methods, agarose electrophoresis (AGE) and cellulose acetate electrophoresis (CAE). Reference intervals in healthy adults were determined for each method. When compared with AGE and CAE, CZE gave substantially higher reference values for the alpha1-globulin fraction. With CZE, within-run ...
متن کاملMulticenter evaluation of the Paragon CZE 2000 capillary zone electrophoresis system for serum protein electrophoresis and monoclonal component typing.
Serum protein electrophoresis and typing of monoclonal components (MCs) are routine but time-consuming and technically demanding assays. We evaluated capillary electrophoresis (Paragon CZE 2000) for automation of the two assays. CZE and cellulose acetate electrophoresis gave similar data on 794 samples. Within-run and between-run CVs were < 2% for albumin and gamma-globulins and 4-7% for alpha ...
متن کاملSerum protein electrophoresis and immunofixation by a semiautomated electrophoresis system.
Semiautomated agarose electrophoresis and immunofixation performed with Hydrasys-Hyrys (Sebia) were compared with conventional, manually performed methods, including cellulose acetate electrophoresis, immunoelectrophoresis, and immunofixation. Reference intervals for agarose electrophoresis with Hydrasys-Hyrys were determined. Within-run imprecision (CV) for fraction quantitation with the semia...
متن کاملOptimization of serum protein separation by capillary electrophoresis.
* Author for correspondence. 2. Lewellen U, McCurdy HH. A novel procedure for the analysis of drugs in whole blood by homegeneous enzyme immunoassay (EMIT). J Anal Toxicol 1988:12:260-4. 3. Manca D, Ferron L, Weber JP. A system for toxicological screening by capillary gas chromatography. Chin Chem 1989;35:601-7. 4. Hawks R, Chiang C, eds. Urine testing for drugs of abuse. NIDARes Monogr 73, Roc...
متن کاملCLINICAL APPLICATIONS Capillary Electrophoresis
Column Zorbax silica, 25 cm 4.6 mm internal diameter (7 m) S5W uncoated silica, 25 cm 4.6 mm internal diameter (5 m) Eluent Hexane}ethyl acetate, 95 : 5 CO2 modified with small amounts of methanol Flow rate 1.6 mL min 1 2 mL min 1 for 4 min, then gradient of 2 up to 5 mL min 1 thereafter held constant Programme Isocratic P"100 atm, T"403C; modifier, 1.5%min 1 from 10% to 30% thereafter held con...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1998
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/44.4.749